ABSTRACT
Objective To investigate the recombination events occurring between HLA-A and-C loci discovered in two Chi-nese families.Methods HLA class I (HLA-A and-B)and II (HLA-DRB1)alleles low resolution typing were typed by pol-ymerase chain reaction-sequence specific oligonucleotide,(PCR-SSO)and PCR-sequence specific primer (PCR-SSP).HLA class I and II high resolution typing was done by sequencing-based typing (SBT).Then the recombination sites were ana-lyzed by family study.Results The results indicates that the recombination events occurred in one family between A*30∶01/32∶01-C*06∶02 and another family between A*11∶01/26∶01-C*07∶06 during meiosis.The recombination both came from fathers and resulted in new HLA haplotypes that were inherited by the children.Conclusion Two HLA-A/C re-combination events occurring between HLA-A and-C loci have been found in two Chinese families,which help further study the mechanisms of HLA recombination.
ABSTRACT
Objective To explore the effect of early rehabilitative intervention on postoperative functional rehabilitation of artificial femoral head replacement,to provide the basis for clinical practice.Methods Sixtyfour patients received artificial femoral head replacement were divided randomly into the rehabilitative group and the control group with 32 patients in each group.The patients in the control group received routine rehabilitation exercises,while the rehabilitative group received rehabilitative intervention after 3 hours of operation.The incidence of complications was compared after 2 weeks,and Harris scores were compared after 6 months.Results The incidence of complications in the rehabilitative group was significantly lower than the control group.Harris scores of the rehabilitative group were significantly better than the control group.Conclusions Early rehabilitative intervention can decrease the incidence of complication and accelerate the functional recovery of affected limbs after artificial head replacement.